Precision-Cut Lung Slices (PCLS) Protocols:
Please do not forget to cite us if you use our protocols
Print the 3D lobe divider (3DLD)
Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations
The manuscript is now available on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.05.10.491312v1
You can download the stl files from the github repository: Click Here.
Decellularization of Human IPF lungs
TGF-β1-induced deposition of provisional extracellular matrix by tracheal basal cells promotes epithelial-to-mesenchymal transition in a c-Jun NH2-terminal kinase-1-dependent manner
Decellularization of Human (Normal, COPD) and Pig
Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration
Mouse lung decellularization
The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential
Deconvolution of PCLS bulk RNASeq using IPF Atlas scRNAseq using Bisque
You can find the code in the GitHub Repository: human PCLS BisqueRNA
This code was produced for publication by first author John Stegmayr: Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts
New and improved RNA isolation protocol for PCLS
Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts
PCLS generation from Human tissues
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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